Composite

Part:BBa_K3734025:Design

Designed by: Xingjun Zhao   Group: iGEM21_CSU_CHINA   (2021-10-01)


GIP-miR21T-GI-GAL4-4XmiR21T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4405
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4300
    Illegal XhoI site found at 1953
    Illegal XhoI site found at 5001
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 899
    Illegal NgoMIV site found at 2455
    Illegal AgeI site found at 3682
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4920
    Illegal BsaI.rc site found at 216
    Illegal BsaI.rc site found at 3079


Design Notes

We use 4XmiR21T at 3'UTR.When you use PCR to clone the fragment, you need to focus on how many times the miR21T repeats.


Source

GIP and miR21T come from human genome.GI is from Arabidopsis thaliana.GAL4 is from Saccharomyces cerevisiae.

References